Tunku Ali works across the private, public, and non-profit sectors in Malaysia. He sits on the Council of Law and Justice of the State of Negeri Sembilan, and is a Brigadier General and Commander of the Territorial Army Regiment of Negeri Sembilan.

He is a Senior Advisor to TPG Capital a global Private Equity firm, representing TPG as Chairman/Director across several portfolio company boards in the education and healthcare sectors in Southeast Asia. Separately, he is Chairman of Astro Malaysia Holdings Berhad, a listed media company, and also Chairman of Taliworks Corporation Berhad, a listed infrastructure services company. He also sits on the board of Bangkok Bank Berhad, and is a Partner at Vynn Capital, an early stage Venture Capital firm with investments across Southeast Asia.

He is Chairman and Founding Trustee of Teach for Malaysia (TFM), President of WWF-Malaysoia, Chairman of Cancer Research Malaysia, Chairman of Yayasan Munarah, and a Trustee of Amanah Warisan Negara (Malaysia's National Heritage Trust). He is Chairman of the Board of Governors of Marlborough College Malaysia, and an International Council Member of Marlborough College, United Kingdom. He Pro Chancellor of a Malaysian public university and director/chairman of two private universities.

He holds a Masters of Public Administration from the John F Kennedy School of Government, Harvard University and a BA (Hons) in History and Social & Political Sciences from the University of Cambridge. In addition, he is an Honorary Bencher of the Inner Temple, United Kingdom.

Previously, Tunku Ali was a Consultant with McKinsey & Company and a Director of Investments with Khazanah Nasional Berhad.